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    Inside In Vitro Fertilisation (IVF) Laboratory at GBR Fertility Centre and Hospitals, Chennai, India

    An IVF laboratory has a crucial role to play in the execution of different processes in In Vitro Fertilization (IVF). An IVF cycle can be categorized in three phases. The first one comprises of processing, stimulation of follicles and egg retrieval. During the second phase, eggs and sperms are cultured to form embryos whereas the third phase includes embryo transfer that is placing the resultant embryos back into the uterus. The second phase is carried out in the IVF laboratory where the sperms and eggs are cultured. Here, the eggs are inseminated using the husband’s sperms in a petri dish and put in an incubator to allow fertilization. The embryos are under constant observation and are cultured till the blastocysts stage which is at day 5. The lab requires maintaining a temperature of 37 to 38 degrees Celsius. The temperature and humidity levels are strictly controlled using advanced set up of devices and machines to ensure that the lab has the purest and most conducive environment. Oocytes and sperm are collected and processed in the laboratory. These are then graded and prepared for fertilization. Difference between a conventional IVF cycle and ICSI procedure seen clearly in an IVF lab is a process that is ICSI is carried out using fewer sperms and in absence of sperms in a semen sample, they are obtained with the help of a biopsy using TESA. During ICSI a sperm in selected and then injected into the egg to obtain a healthy embryo. An IVF lab is equipped with a number of devices and equipment and needs to be maintained on a continuous basis.

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    Basics of In Vitro Fertilisation (IVF)

    IVF procedure was started decades back and the first baby who was born was named Louis Brown. A couple needs to opt for IVF procedure in case of infertility issues in couples. Certain IVF injections and medications are given to the woman for conception. IVF procedure involves specific steps which lead to the formation of embryo. Embryos in the cleavage stage are transferred to the uterus of the woman. Some of the doctors suggest that embryo needs to be cultured for long, so that there are increased chances of pregnancy despite having lesser number of embryos for implantation. Proper monitoring of the procedure is done to make sure that the patients do not have any kind of complications during the procedure in the form of unwanted multiple pregnancies after implantation. Also, Natural IVF procedure is performed to make sure that the patients receive quality treatment with desired results. The usage of medications is reduced in case of Natural Cycle IVF. Thus, IVF procedure boosts chances of successful pregnancy in couples.

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    How does vitro fertilization (IVF) works?

    IVF procedure includes most of the steps which are involved in the natural reproduction procedure. First, follicle matures and with the LH surge follicle is released. The egg is directed into the Fallopian tube. Then, if the egg is not fertilized within 24 hours with the help of sperm, then the procedure is repeated. Only one sperm can enter the egg and fertilize it. If fertilization occurs, then the embryo is implanted in the interior lining of the uterus of woman. It takes around 3 days for the implantation to take place after the formation of the embryo. In IVF, FSH is administered to the woman with assistance of ultrasound. In some cases, sperm reaches the egg and enters it for embryo formation. In some cases, certain additional steps are performed where the embryologist injects sperm into the egg which make it important for the utilization of procedures such as ICSI. In certain cases, donor eggs are also used. IVF success rates are the highest among most of the infertility treatments.

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    Treatment process of In Vitro Fertilisation (IVF)

    In Vitro Fertilization (IVF) is one of the top assisted reproductive technologies for conceiving a baby. In the IVF treatment, certain number of eggs are removed from the ovaries of the woman. Final maturation of the eggs are performed with the utilization of medications for the same. The woman is given with light sedation for retrieval of the eggs after maturation. Under the guidance of the ultrasound, eggs are removed from the woman’s body. The washed and concentrated sperms are taken from the male partner or donor which leads to formation of embryo. This embryo is transferred to the uterus of the woman with the assistance of a thin flexible tube which reaches inside the uterus of the female partner in the couple. The success rates of IVF treatment is impressive. The demand of IVF procedure has increased over the years and therefore infertility specialists advice couples to opt for the same to conceive a baby.

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