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    Inside an In Vitro Fertilisation (IVF) Laboratory

    IVF laboratory in an infertility centre comprises of modern medical procedures which are performed under the guidance of the medical experts and embryologists. Some of the important medical procedures which are performed in the IVF laboratory includes Intracytoplasmic Sperm Injection (ICSI), Trophectoderm Biopsy, and Embryo freezing procedures. When the samples are taken from the couples, then they are marked with the assistance of unique ids and names to recognize at the time of their infertility treatment. The culture dishes are one of the vital parts of the IVF laboratory with the help of which the samples are stored in the incubator and the procedure is performed. Conventional insemination procedure is performed in the IVF laboratory. Here, the embryologist places the sperm beside the egg. The sperm penetrates into the egg on its own and the fertilization takes place. Cleavage assessment is performed under the guidance of the embryologist which make sure that only quality embryo is used for the implantation procedure. Embryologist also performed the procedure in which the sperm is injected in the egg which make sure that the fertilization is induced. Cryopreservation is also performed in the IVF laboratory.

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    How does vitro fertilization (IVF) works?

    IVF procedure includes most of the steps which are involved in the natural reproduction procedure. First, follicle matures and with the LH surge follicle is released. The egg is directed into the Fallopian tube. Then, if the egg is not fertilized within 24 hours with the help of sperm, then the procedure is repeated. Only one sperm can enter the egg and fertilize it. If fertilization occurs, then the embryo is implanted in the interior lining of the uterus of woman. It takes around 3 days for the implantation to take place after the formation of the embryo. In IVF, FSH is administered to the woman with assistance of ultrasound. In some cases, sperm reaches the egg and enters it for embryo formation. In some cases, certain additional steps are performed where the embryologist injects sperm into the egg which make it important for the utilization of procedures such as ICSI. In certain cases, donor eggs are also used. IVF success rates are the highest among most of the infertility treatments.

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    Are you a candidate for IVF Treatment?

    Patients or couples who have been trying for a baby for more than a year are suspected for infertility and can go for infertility treatment that can include IUI and fertility medication. A couple can consider themselves to be a right candidate for IVF (In Vitro Fertilization) when they have experienced failed fertility treatments in the past like IUI or Intrauterine Insemination. After few cycles of IUI fail, a couple should start considering IVF as the next treatment option. For those women who have the issue of blocked fallopian tubes, IVF has to be the first choice of treating their infertility as blocked tubes do not facilitate fertilization of egg and sperm inside the tube. Women who suffer from severe endometriosis have IVF as their treatment option. It is a condition when the tissue lining of the endometriosis grows uncontrollably inside and outside of the uterus making it almost impossible to conceive naturally. IVF can work effectively for those suffering from endometriosis. Then there are those couples who have experienced previous IVF cycles and have not succeeded so far. Such couples can continue to repeat IVF cycles as the chances improve with every attempt.

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    Inside In Vitro Fertilisation (IVF) Laboratory at GBR Fertility Centre and Hospitals, Chennai, India

    An IVF laboratory has a crucial role to play in the execution of different processes in In Vitro Fertilization (IVF). An IVF cycle can be categorized in three phases. The first one comprises of processing, stimulation of follicles and egg retrieval. During the second phase, eggs and sperms are cultured to form embryos whereas the third phase includes embryo transfer that is placing the resultant embryos back into the uterus. The second phase is carried out in the IVF laboratory where the sperms and eggs are cultured. Here, the eggs are inseminated using the husband’s sperms in a petri dish and put in an incubator to allow fertilization. The embryos are under constant observation and are cultured till the blastocysts stage which is at day 5. The lab requires maintaining a temperature of 37 to 38 degrees Celsius. The temperature and humidity levels are strictly controlled using advanced set up of devices and machines to ensure that the lab has the purest and most conducive environment. Oocytes and sperm are collected and processed in the laboratory. These are then graded and prepared for fertilization. Difference between a conventional IVF cycle and ICSI procedure seen clearly in an IVF lab is a process that is ICSI is carried out using fewer sperms and in absence of sperms in a semen sample, they are obtained with the help of a biopsy using TESA. During ICSI a sperm in selected and then injected into the egg to obtain a healthy embryo. An IVF lab is equipped with a number of devices and equipment and needs to be maintained on a continuous basis.

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