in vitro fertilisation (ivf)

Understanding the procedure of IVF - 6 steps to achieve IVF pregnancy

  • 4.8   (6 Votes)
Medically Reviewed by Dr. Deepika Tiwari - MBBS on 19 Dec 2018 - Written by Dr. Sailaja Vuppu - MBBS - Grammatically Approved by Dr. Kavita Jaggi Agrawal - MBBS

The six basic IVF procedure steps include ovarian stimulation, progress check, egg collection, fertilization, embryo transfer, and successful implantation. Your infertility specialist will perform IVF procedure step by step and advice certain IVF treatment success tips to achieve IVF treatment results.

When planning for a baby through fertility treatment, IVF treatment or IVF procedure and test tube baby or In Vitro Fertilization is one of the most effective ones available these days. However, not many of us know what exactly it is and how it works. Here is a step-b-step detailed description of the IVF treatment or IVF procedure and test tube baby.

Six Steps of IVF from Conception to Completion:

  1. Ovary Stimulation
  2. Progress Check
  3. Collection Of Eggs
  4. Fertilization
  5. Embryo Transfer
  6. Successful Implantation

How is IVF Performed

1. Ovary Stimulation: 

8 to 14 days prior to the next menstrual cycle, the woman is given medicines to suppress her natural cycle. (To know your next predicted period dates, refer Ela App). The natural cycle is suppressed through daily injections, tablets or a nasal spray. After suppressing the natural cycle, fertility drugs are given to the woman for about 2 weeks, in order to stimulate the ovaries. These drugs help in developing multiple matured eggs instead of just one. Along with these, the woman has to take synthetic hormones to keep her body from releasing matured eggs too early.

2. Progress Check:

Every woman responds to these fertility drugs differently and the doctor keeps an eye on possible side-effects. The doctor will monitor the progress of matured eggs through vaginal ultrasound scans and blood tests. Within 36 hours of collecting the eggs, a hormone injection is given to help the eggs grow in a positive environment. 

test tube baby, ivf

3. Collection Of Eggs:

By giving an anesthesia to the woman, an ultrasound probe is inserted to examine the follicular development in each ovary. A thin needle is inserted to remove the eggs from the ovaries. One might experience some discomfort in terms of cramps or spotting but these symptoms disappear in a day or two. 

4. Fertilization:

An embryologist (a scientist who specializes in eggs, sperm, and embryos) examines the eggs before combining them with the sperm. The eggs are mixed with the sperm and cultured in the laboratory for about 16-20 hours. In the next step, the eggs are checked for fertilization. The fertilized eggs (now called as embryos) will be placed in an incubator for up to six days and the development of the embryos is closely monitored. The embryos are then examined and the healthiest embryo is selected for transferring. The remaining healthy embryos are stored and freezed for future IVF treatment or IVF procedure and test tube baby if needed. 

ivf treatment, ivf procedure

5. Embryo Transfer:

Depending on the couple’s age and severity of the fertility problem, doctor places between one to five embryos in the uterus by inserting a thin tube (a catheter) through the cervix. The number of embryos transferred is restricted because of the risks associated with multiple births. Due to this, the doctor might recommend single embryo transfer (SET) if he/she feels it is the best option for you. This is usually a pain-free procedure and no sedation is necessary, but one may experience a little discomfort as you need a full bladder in case of ultrasound.

6. Successful Implantation:

If the treatment works perfectly, then the next step involves implanting an embryo in the uterine wall. One can take the pregnancy test about two weeks after the insertion of the embryo. 

Whether I should go for IUI or IVF treatment? Confused! To clear your doubts, please read our article on IUI or IVF. In case you are looking for the best IVF treatment then explore our article on Best IVF Doctors in Delhi With High Success Rates.

Dr. Sailaja Vuppu

Written by

Infertility Specialist, Gynecologic Surgeon

Dr Sailaja Vuppu is an acclaimed Gynaecologist and Infertility Specialist in Sri Ramachandra Nagar, Vijayawada. She has completed 10 years on working as a gynaecologist and infertility specialist. Dr Sailaja Vuppu is right now practising at Rainbow Super Speciality Hospital for Women and Children. Her fields of expertise are Hysterectomy (Abdominal/Vaginal), Intracytoplasmic Sperm Injection (ICSI), Intrauterine Insemination (IUI), In-Vitro Fertilization (IVF) and so on. Find her contact details on


  • In vitro fertilization (ivf) - process, requirement, success rate, risks and cost  In Vitro Fertilization, Elawoman - 2017
  • Facts you must know about embryo freezing - procedure, benefits, disadvantages  embryos, Elawoman - 2017
  • Facts you must know about embryo freezing - procedure, benefits, disadvantages  stored and freezed, Elawoman - 2017



Kajal Prajapati

Very useful information for the childless couples who consider IVF as the best alternative.

Aug. 26, 2018, 2:43 p.m. 4.5


Daisy Frank

Being an alcoholic I got a failure in the first attempt of IVF. This time I am not going to take any chance. I have quit smoking and drinking.

Feb. 1, 2018, 8:48 a.m. 5.0


Mohammad Imran

The intended mother should eat a healthy diet and take proper sleep to increase the chances of success of the IVF cycle.

Nov. 3, 2017, 5:02 p.m. 5.0


Anahita Sharma

Test tube baby through IVF treatment has given lives to many infertile couples. It's a proven boon in the field of medicines and treatment.

Aug. 31, 2017, 5:14 p.m. 4.5


Sonia Rai

Alcohols and smoking should be avoided while planning pregnancy. It will also help in healthy working of IVF procedure.

Aug. 31, 2017, 5:12 p.m. 4.5


Mohammad Gulfaan

IVF treatment can even help those women who cannot conceive because of breasts or uterine cancer. They can also be benefited with this treatment.

Aug. 31, 2017, 4:08 p.m. 5.0

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